UDP and CDP Nucleotide Sugars Analyzed with LC-MS MS - AppNote
May 15, 2012
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Date: 15-MAY-2012 Last Updated: 22-AUGUST-2026
Separating UDP and CDP Sugars with HILIC Increased Sensitivity
This Method can be used to analyze UDP and CDP Sugars using UDP-Hexanolamine (a metabolite) as an Internal standard. The Sugar Nucleotides used in this Application Note are a mixture of compounds that occur in plants and their structure is proprietary.A potentially powerful tool for profiling Sugar Nucleotides in Metabolomic studies, this Method uses an Inverse Gradient (HILIC like); the Mobile Phase uses high organic component which enhances Mass Spec response and assures lower detection limits.
Method Conditions
Column: Cogent Diamond Hydride™, 4 μm, 100 Å
Catalog No.: 70000-15P-2
Dimensions: 2.1 x 150 mm
Mobile Phase:
-- A: DI Water / 0.1% Ammonium Formate (pH 7.2)
-- B: 90% Acetonitrile / 10% DI Water / 0.1% Ammonium Formate (pH 6)
Gradient:
|
Time (minutes) |
%B |
|---|---|
| 0 | 95 |
| 10 | 75 |
| 12 | 75 |
| 12.1 | 95 |
| 15 | 95 |
Post Time: 5 minutes
Flow rate: 0.3 mL / minute
Detection: ESI – neg – Agilent 6410 Triple Quadrupole Mass Spectrometer
Mass Data:
- 1. Compound 1 - the monitored MRM transitions were m/z 535 to m/z 323
- 2. Compound 2 - the monitored MRM transitions were m/z 564 to m/z 322
- 3. UDP Hexanolamine (internal standard) - the monitored MRM transitions were m/z 502 to m/z 258 (MRM = multiple reaction monitoring in LC/MS/MS)
Notes: Sugar Nucleotides among other metabolites are an important group of compounds to be analyzed when one is trying to understand cellular response to genetic or environmental perturbations.
